“This is a brilliant book and touches on every aspect of biotechnology.” Amazon review
Even as biotechnology advances dominate the general and scientific news, the fundamental practices of quality laboratory work have not changed. Basic Laboratory Methods for Biotechnology, Third Edition, is both a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry, and a valuable reference for laboratory professionals at all stages of their careers. Basic laboratory skills are emphasized, within the context of modern innovations. There is an underlying theme of “reproducibility” that informs the descriptions and examples provided. Tables and boxed content provide step by step information for ease of reference, while practice problems and solutions, case studies, and anecdotes provide useful illustrations of common issues in the laboratory and beyond.
Editorial review:
“This hefty, 1,171-page, paperback book is worth its weight in gold. What a fantastic reference for the various aspects of biotechnology. I loved the emphasis on the “relationship between proper fundamental practices and reproducibility,” and the authors’ steadfast commitment to providing a “solid grounding in basic, quality practices” essential for success in biotechnology. The sheer size and comprehensiveness of this book reinforces their introductory statement, “..’biotechnology’ is not a synonym for ‘molecular biology.'” Pretty much every possible test method used in a biotechnology setting (e.g., cell culture, chromatography, immunoassay) and their essential quality components (e.g., measurements such as weight and temperature; and solutions – love the declarative statement for section 24.1.1.2: “water is not pure”) are described in great detail. Case studies accompany each chapter for the learners to test their retention and understanding. A nice inclusion is the section on regulatory affairs for learners to appreciate the challenges of bringing a biotechnology product to the consumer. This is a fabulous textbook. If you work in biotechnology, this should be on every workbench and administrative office (e.g., supervisors, regulatory, quality assurance) for easy reference and, most importantly, adherence to quality practices.”
Valerie L Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Purchase Basic Laboratory Methods for Biotechnology or request an inspection copy
Sample chapter available (Chapter 20: The Measurement of pH, Selected Ions, and Conductivity)
Table of Contents
UNIT I: INTRODUCTION: BIOTECHNOLOGY IS THE TRANSFORMATION OF KNOWLEDGE INTO USEFUL PRODUCTS
Chapter 1: Techniques to Manipulate DNA: The Root of the Biotechnology Industry
Chapter 2: The Biotechnology Industry Branches Out
Chapter 3: The Business of Biotechnology: The Transformation of Knowledge into Products
UNIT II: INTRODUCTION TO QUALITY IN BIOTECHNOLOGY WORKPLACES
Chapter 4: An Overview of Product Quality Systems in Biotechnology
Chapter 5: Quality in Research Laboratories
Chapter 6: Documentation: The Foundation of Quality
UNIT III: SAFETY IN THE LABORATORY
Chapter 7: Introduction to a Safe Workplace
Chapter 8: Working Safely in the Laboratory: General Considerations and Physical Hazards
Chapter 9: Working Safely with Chemicals
Chapter 10: Working Safely with Biological Materials
UNIT IV: MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW
Chapter 11: Basic Math Techniques
Chapter 12: Proportional Relationships
Chapter 13: Relationships and Graphing
Chapter 14: Descriptions of Data: Descriptive Statistics
UNIT V: OBTAINING REPRODUCIBLE LABORATORY MEASUREMENTS
Chapter 15: Introduction to Quality Laboratory Measurements
Chapter 16: Introduction to Instrumental Methods and Electricity
Chapter 17: The Measurement of Weight
Chapter 18: The Measurement of Volume
Chapter 19: The Measurement of Temperature
Chapter 20: The Measurement of pH, Selected Ions, and Conductivity
Chapter 21: Measurements Involving Light A: Basic Principles and Instrumentation
UNIT VI: LABORATORY SOLUTIONS
Chapter 22: Preparation of Laboratory Solutions A: Concentration Expressions and Calculations
Chapter 23: Preparation of Laboratory Solutions B: Basic Procedures and Buffers
Chapter 24: Solutions: Associated Procedures and Information
Chapter 25: Laboratory Solutions to Support the Activity of Biological Molecules
UNIT VII: QUALITY ASSAYS AND TESTS
Chapter 26: Introduction to Quality Laboratory Tests and Assays
Chapter 27: Achieving Reproducible PCR Assay Results
Chapter 28: Measurements Involving Light B: Assays
Chapter 29: Achieving Reproducible Immunoassay Results
UNIT VIII: CELL CULTURE AND REPRODUCIBILITY
Chapter 30: Introduction to Quality Practices for Cell Culture
Chapter 31: Culture Media for Intact Cells
UNIT IX: BASIC SEPARATION METHODS
Chapter 32: Introduction to Filtration
Chapter 33: Introduction to Centrifugation
Chapter 34: Introduction to Bioseparations
UNIT X: BIOTECHNOLOGY AND REGULATORY AFFAIRS
Chapter 35: Biotechnology and the Regulation of Medical and Food Products
Chapter 36: The Lifecycle and Regulation of Pharmaceutical Products
Chapter 37: The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products
Chapter 38: Quality Systems in a Regulated Production Facility
Appendix: Answers to Practice Problems
Acronyms and Glossary
Purchase Basic Laboratory Methods for Biotechnology or request an inspection copy
Sample chapter available (Chapter 20: The Measurement of pH, Selected Ions, and Conductivity)